female coronary artery ecs  (ATCC)

Journal: bioRxiv

Article Title: Mitochondrial Dysfunction in Endothelial Cells Drives Greater Vascular Impairment in Females with Diabetes-Associated Peripheral Artery Disease

doi: 10.1101/2025.09.22.677648

Figure Lengend Snippet: (A) Schematic illustrating site of tissue collection from below- and above knee amputations. (B) Myography showing increased endothelial dysfunction in female vs male patient arteries. Left , endothelial-dependent relaxation in response to acetylcholine (ACh). Right , No difference in vascular smooth muscle cell (VSMC), sodium nitroprusside (SNP)- mediated relaxation (n=3-5). (C) Left, representative image of microvessels in tibialis anterior (CD31 + SMA + , yellow arrows). Laminin (myocytes, white), CD31 (ECs, red) and SMA (pericytes, green); scale bar 50 μm. Right , quantification (n=5/sex). (D) Myocyte area is unaltered with sex (n=5). (E) NADPH oxidase ( Nox ) mRNA marker expression in muscle measured by qPCR, normalized to β -actin (n=5). (F) 8-isoprostane levels in plasma remain unchanged with sex (n=5). Results are mean±SEM; two-way ANOVA, Student’s t -test or Mann–Whitney U -test; * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Primary human male and female coronary artery ECs were purchased from American Type Culture Collection.

Techniques: Marker, Expressing, Clinical Proteomics, MANN-WHITNEY

Journal: bioRxiv

Article Title: Mitochondrial Dysfunction in Endothelial Cells Drives Greater Vascular Impairment in Females with Diabetes-Associated Peripheral Artery Disease

doi: 10.1101/2025.09.22.677648

Figure Lengend Snippet: (A) Schematic to show vessel collection for myography. (B) Myography showing increased endothelial dysfunction in female vs male arteries. Left , endothelial-dependent relaxation in response to acetylcholine (ACh). Right , sodium nitroprusside (SNP)-mediated relaxation (n=5-7). (C) Myography in non-injured vessels (N=5-7). (D) Plasma nitrite/nitrate levels (n=4-6). Laser Doppler imaging showing reduced blood perfusion over time with diabetes in (E) male and (F) female mice. Left, representative image of blood flow at 14 d. Right, quantification. (n=9-11). (G) Laser doppler perfusion index at 14 days (n=9-11). (H) Microvessel number (CD31+SMA+, <50 µm in diameter) in gastrocnemius tissues normalized to the number of myocytes and control non-ischemic limbs (n=9-11). (I) Tubule formation of male and female murine ECs shows an altered phenotype. (J) Tubulogenesis is reduced in female ECs under diabetic conditions. Left, Wimasis platform for vessel coverage and networks. Right , quantification (n=5/group). Results are mean±SEM; two-way ANOVA, Student’s t -test or Mann–Whitney U -test; * P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001.

Article Snippet: Primary human male and female coronary artery ECs were purchased from American Type Culture Collection.

Techniques: Clinical Proteomics, Imaging, Control, MANN-WHITNEY

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: EC and SMC cell coverage on flat and NT surfaces. (A) EC and (E) SMC cell numbers on flat and NT surfaces were measured using a CyQUANT assay, which quantifies DNA content in cell lysates. (B) EC and (F) SMC area were quantified using fluorescence microscopy and ImageJ. Representative images of (C,D) ECs and (G,H) SMCs cultured on flat and NT surfaces are shown. Green: phalloidin. Blue: DAPI. Scale bar: 150 μm.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: CyQUANT Assay, Fluorescence, Microscopy, Cell Culture

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Total FAK and pFAK were quantified by ELISA using cell lysates from (A,B) ECs and (C,D) SMCs cultured on flat and NT90 surfaces. Total FAK concentration was normalized to the total protein content. The pFAK concentration was normalized to the total FAK concentration. Data are expressed as mean ± SD.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Concentration Assay

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Effect of NT topography on inflammatory response. (A) VCAM-1 gene expression in ECs. (B) SMC cell numbers when cultured on flat or NT90 surfaces in control media or under stimulation with 2 ng/mL TNFα, an inflammatory cytokine and known mitogen for SMCs. (C) MCP-1 secretion by SMCs cultured on flat or NT surfaces was measured in conditioned media using ELISA. Data are expressed as mean ± SD.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Effect of varying NT diameter on EC and SMC response. Cell growth rates on flat and NT surfaces using (A) ECs and (D) SMCs. Significant values are represented as follows: x NT30; # NT50; *NT90. One symbol: p < 0.05; two symbols: p < 0.01. (B) Relative VCAM-1 mRNA expression in ECs cultured on flat and NT surfaces. Values are normalized to mRNA expression on flat surfaces. (C) SMC cell numbers cultured on flat and NT surfaces in control media and in media containing 2 ng/mL TNFα. (E) MCP-1 secretion by SMCs cultured on flat and NT surfaces. Note: data shown here for flat and NT90 surfaces are identical as those in previous figures. They are shown here as a reference for comparison purposes.

Article Snippet: Cell Culture and Cell Proliferation Assays Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: EC and SMC cell coverage on flat and NT surfaces. (A) EC and (E) SMC cell numbers on flat and NT surfaces were measured using a CyQUANT assay, which quantifies DNA content in cell lysates. (B) EC and (F) SMC area were quantified using fluorescence microscopy and ImageJ. Representative images of (C,D) ECs and (G,H) SMCs cultured on flat and NT surfaces are shown. Green: phalloidin. Blue: DAPI. Scale bar: 150 μm.

Article Snippet: Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: CyQUANT Assay, Fluorescence, Microscopy, Cell Culture

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Total FAK and pFAK were quantified by ELISA using cell lysates from (A,B) ECs and (C,D) SMCs cultured on flat and NT90 surfaces. Total FAK concentration was normalized to the total protein content. The pFAK concentration was normalized to the total FAK concentration. Data are expressed as mean ± SD.

Article Snippet: Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Concentration Assay

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Effect of NT topography on inflammatory response. (A) VCAM-1 gene expression in ECs. (B) SMC cell numbers when cultured on flat or NT90 surfaces in control media or under stimulation with 2 ng/mL TNFα, an inflammatory cytokine and known mitogen for SMCs. (C) MCP-1 secretion by SMCs cultured on flat or NT surfaces was measured in conditioned media using ELISA. Data are expressed as mean ± SD.

Article Snippet: Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: ACS biomaterials science & engineering

Article Title: TiO 2 -Based Nanotopographical Cues Attenuate the Restenotic Phenotype in Primary Human Vascular Endothelial and Smooth Muscle Cells

doi: 10.1021/acsbiomaterials.9b01475

Figure Lengend Snippet: Effect of varying NT diameter on EC and SMC response. Cell growth rates on flat and NT surfaces using (A) ECs and (D) SMCs. Significant values are represented as follows: x NT30; # NT50; *NT90. One symbol: p < 0.05; two symbols: p < 0.01. (B) Relative VCAM-1 mRNA expression in ECs cultured on flat and NT surfaces. Values are normalized to mRNA expression on flat surfaces. (C) SMC cell numbers cultured on flat and NT surfaces in control media and in media containing 2 ng/mL TNFα. (E) MCP-1 secretion by SMCs cultured on flat and NT surfaces. Note: data shown here for flat and NT90 surfaces are identical as those in previous figures. They are shown here as a reference for comparison purposes.

Article Snippet: Primary human coronary artery ECs and primary human coronary artery SMCs were purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture

Journal: The Journal of Clinical Investigation

Article Title: Endothelial cell α -globin and its molecular chaperone α -hemoglobin–stabilizing protein regulate arteriolar contractility

doi: 10.1172/JCI99933

Figure Lengend Snippet: (A) Human coronary ECs were transduced with lentiviral expression vectors encoding human α-globin–GFP, human AHSP-mCherry, or human eNOS-mCherry fusion proteins and visualized by fluorescence microscopy. Representative images of merged GFP and mCherry signals in single cells are shown (white rectangles and far-right panels). Scale bars: 25 μm (panels in first 3 columns) and 10 μm (panels at far right). (B) Western blot detection of the indicated proteins in cells shown in A. The indicated proteins are as follows: 1, eNOS-mCherry; 2, endogenous eNOS; 3, AHSP-mCherry; 4, recombinant human AHSP (2 ng); 5, α-globin–GFP; and 6, purified α-globin (2 ng). (C) Quantification of α-globin–GFP protein expression by Western blotting as in B (n = 3 independent experiments). (D) Quantitative RT-qPCR analysis of α-globin–GFP mRNA 4 days after lentiviral transduction with vectors encoding the indicated proteins (n = 4 independent experiments). *P < 0.05 and **P < 0.01, by unpaired t test.

Article Snippet: Human primary coronary ECs (Lonza) were grown in EGM-2 Bullet Kit Medium (Lonza).

Techniques: Transduction, Expressing, Fluorescence, Microscopy, Western Blot, Recombinant, Purification, Quantitative RT-PCR